Journal: Advanced Healthcare Materials

Article Title: Bioactive Suture with Added Innate Defense Functionality for the Reduction of Bacterial Infection and Inflammation

doi: 10.1002/adhm.202300987

Figure Lengend Snippet: Human neutrophil elastase‐induced TCP‐25 fragmentation in TCP‐25 sutures. a) Peptide fragmentation pattern of TCP‐25 after treatment of TCP‐25 suture with human neutrophil elastase. TCP‐25 suture was incubated with human neutrophil elastase and analyzed by nano LC‐MS/MS. The figure shows the sequences of main peptides and the quantity of successful identifications by mass spectrometry at 0, 30, and 180 min ( n = 2). b) Illustration of main peptides obtained after human neutrophil elastase digestion of TCP‐25 from coated sutures. *These peptides have been reported to show antibacterial effects. c) Antibacterial activity of peptide fragment products obtained after treatment of TCP‐25 suture with human neutrophil elastase was analyzed by evaluating the antibacterial activity against E. coli by RDA. The bar chart shows quantification of the clear zone. Data are shown as the mean ± SEM ( n = 3). d) The anti‐inflammatory activity of peptide fragment products obtained after treatment of TCP‐25 suture with human neutrophil elastase. NF‐κB and AP‐1 activation was evaluated in THP1‐Xblue‐CD14 reporter cells. Data are shown as the mean ± SEM ( n = 3). *** P ≤ 0.001. NS: non‐significant.

Article Snippet: To investigate further if TCP‐25 coated sutures exhibit in vivo antibacterial efficacy against Escherichia coli , sutures were contaminated with CLSI control strain E. coli (ATCC 25922), and tissue around the suture site was analyzed at 72 h. CFU determination showed that TCP‐25‐coated sutures caused a significant reduction in number of bacteria (Figure , Supporting Information).

Techniques: Incubation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Activity Assay, Activation Assay

Journal: Advanced Healthcare Materials

Article Title: Bioactive Suture with Added Innate Defense Functionality for the Reduction of Bacterial Infection and Inflammation

doi: 10.1002/adhm.202300987

Figure Lengend Snippet: Determination of the tensile strength, hemolytic activity, and effects upon long‐term storage of TCP‐25 sutures. a) Effect of TCP‐25 coating on the tensile strength of the sutures. The tensile strengths of freshly coated non‐implanted (left panel) or tissue‐implanted (right panel) sutures were measured. To study the effect of TCP‐25 coating on the tensile strength in vivo, sutures were subcutaneously implanted in mice for 4 days. Data are shown as the mean ± SEM ( n = 5). A Mann–Whitney U test was used to calculate P values. b) Hemolytic activity of control and TCP‐25‐coated sutures. A hemolysis assay was performed using human blood. The left bar chart shows the hemolytic activity of 1 cm long sutures, and the right bar chart shows the hemolytic activity of 10 cm long sutures (mean values ± SEM are presented, n = 3). NS: non‐significant. c) HPLC analysis of control (fresh TCP‐25) or TCP‐25 eluted from coated sutures after long‐term storage. TCP‐25 sutures were stored at room temperature for 18 months after which peptides were eluted for HPLC analysis. d) Western blot analysis of TCP‐25 eluted from coated sutures after 18 months of storage at room temperature. e) Results showing CD spectra of TCP‐25 from stored sutures with and without LPS. Fresh TCP‐25 was used as a control for comparison. f) Suture storage effect on the antimicrobial activity and release of TCP‐25 as evaluated by radial diffusion assay using E. coli . Results show quantification of the clear zones. Data are shown as the mean ± SEM ( n = 3–4). NS: non‐significant.

Article Snippet: To investigate further if TCP‐25 coated sutures exhibit in vivo antibacterial efficacy against Escherichia coli , sutures were contaminated with CLSI control strain E. coli (ATCC 25922), and tissue around the suture site was analyzed at 72 h. CFU determination showed that TCP‐25‐coated sutures caused a significant reduction in number of bacteria (Figure , Supporting Information).

Techniques: Activity Assay, In Vivo, MANN-WHITNEY, Control, Hemolysis Assay, Western Blot, Circular Dichroism, Comparison, Diffusion-based Assay